Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1994-10-27
1998-08-18
Ulm, John
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352523, 4353201, C12N 1500, C12N 120
Patent
active
057957525
ABSTRACT:
Disclosed is a method of producing a complementary DNA (cDNA) library enriched in ligand-inducible genes of a cell. The method includes activating a cellular ligand receptor for a predetermined period of time in the presence of labelled RNA precursors and a substance which enhances the level of RNA in said cell. The RNA presursors are incorporated into the RNA synthesized by said cell in response to receptor activation. The labelled RNA is separated from unlabelled RNA and used to prepare cDNA. The cDNA is cloned into host cells to provide a cDNA library of cDNA-containing clones. This library can then be screened for clones containing ligand-inducible genes.
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Amzel Vivana
Trustees of Dartmouth College
Ulm John
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