Method for creating polynucleotide and polypeptide sequences

Details Method for creating polynucleotide and polypeptide sequences Method for creating polynucleotide and polypeptide sequences

2007-01-23

2007-01-23

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

C435S006120, C536S023100, C536S024300

Reexamination Certificate

active

10371168

ABSTRACT:
The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.

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