Liquid purification or separation – Processes – Separating
Patent
1996-07-08
1998-07-28
Kim, John
Liquid purification or separation
Processes
Separating
210787, 210806, 435 2, 436177, B01D 2126
Patent
active
057858691
ABSTRACT:
A method and apparatus for creating a leukocyte rich sample from a mixed population of blood cells. The method comprises the steps of: separating an amount of blood into layers that include a buffy coat layer having a mixed population of blood cells including plasma and red blood cells; providing a chamber having an opening for receiving a fluid; causing the buffy coat layer to enter the chamber; centrifuging the chamber to separate the buffy coat layer into at least a plasma containing layer, a leukocyte containing layer, and a red blood cell containing layer; and separating the leukocyte containing layer from the other layers in the chamber.
REFERENCES:
patent: 4223672 (1980-09-01), Terman et al.
patent: 5071570 (1991-12-01), Shiraki et al.
patent: 5089146 (1992-02-01), Carmen et al.
patent: 5102407 (1992-04-01), Carmen et al.
patent: 5154716 (1992-10-01), Bauman et al.
patent: 5236716 (1993-08-01), Carmen et al.
patent: 5342753 (1994-08-01), Smith, Jr.
patent: 5399268 (1995-03-01), Pall et al.
patent: 5472621 (1995-12-01), Matkovich et al.
patent: 5686238 (1997-11-01), Martinson et al.
Barbara, John A.J. et al. "Polymerase Chain Reaction and Transfusion Microbiology" in Vox Sang 1993: 64: pp. 73-81.
B.o slashed.yum, Arne. "Separation of Lymphocytes, Granulocytes, and Monocytes from Human Blood Using Iodinated Density Gradient Media" in Methods in Enzymology, vol. 108, 1984, pp. 88-102.
Bruisten, S.M. et al. "Efficiency of White Cell Filtration and a Freeze-Thaw Procedure for Removal of HIV-Infected Cells From Blood" in Transfusion, vol. 30, No. 9, 1990, pp. 833-837.
Frewin, D.B. et al. "A Comparative Study of the Effect of Three Methods of Leukocyte Removal on Plasma Histamine Levels in Stored Human Blood" in Seminars in Hematology, vol. 28, No. 3, Suppl. 5, Jul. 1991, pp. 18-21.
Harlowe et al. "Antibodies: A Lab Manual" Cold Spring Harbor, 1988, pp. 584-589.
Hogman, Claes F. et al. "The Bottom and Top System: A New Technique for Blood Component Preparation and Storage"in Vox Sang 1988, 55, pp. 211-217.
Keller, George H. et al. "DNA Probes: Background, Applications, Procedures" MacMillan Publishers, 1993, pp. 36-39; 385-403.
Lueptow, Richard M. et al. "Sedimentation of a Suspension in a Centrifugal Field" Dept. Of Mechanical Engineering. Northwestern University, Evanston IL pp. 1-23, (undated).
Maniatis, T. et al. "Molecular Cloning: A Laboratory Manual" Cold Spring Harbor Laboratory, 1982, pp. 79-83.
Perrow, Jenny G. "The Preparation of Leucocyte Homogenates for Enzymatic Assays" in New Zealand Medical Lab. Technol., Mar. 1977, pp. 6-9.
Rawal, Bhupat D. et al. "Evaluation of Leukcyte Removal Filters Modelled by Use of HIV-Infected Cells and DNA Amplification" in Blood, vol. 76, Nov. 15, 1990, pp. 2159-2161.
Sutton, Don W. et al. "Cell Separation in the Buffy Coat"in Biorheology, 25, 1988, pp. 663-673.
Bratten William
Chapman John
Martinson Jeffrey
Wang Li Ming
Barrett Robert M.
Baxter International Inc.
Kim John
Price Bradford R. L.
Serewicz Denise M.
LandOfFree
Method for creating a leukocyte rich sample from a mixed populat does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for creating a leukocyte rich sample from a mixed populat, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for creating a leukocyte rich sample from a mixed populat will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-20213