Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Patent
1995-06-01
1997-05-06
Rollins, John W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
210295, 210615, 435 29, 435 39, 435243, 435291, 435297, 435300, 435301, C02F 300, C12Q 106, C12Q 166
Patent
active
056270429
ABSTRACT:
A rapid, convenient, and highly sensitive method for counting the number of living microorganisms is disclosed, wherein a test solution is filtered through a membrane filter element having a plurarity of hydrophilic sections separated from each other by hydrophobic partitions. A microparticulate spray is then applied to the filter in order to add extracting and luminescence-inducing reagents in such an amount that allows the filter membrane within each section to become wet. Further, the sample obtained is processed by applying a highly sensitive luminescence image analyzing system thereto. The employment of a spray method for applying the extracting and luminescence-inducing reagents allows entrapment of the reagents and extracted substances within each of the hydrophilic sections. Therefore, when combined with the use of a luminescence image analyzing system, the number of bright spots so produced of which represent the living microorganisms may be rapidly counted without the requirement of cultivation.
REFERENCES:
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patent: 4587213 (1986-05-01), Malecki
patent: 4908236 (1990-03-01), Pitt et al.
patent: 5366867 (1994-11-01), Kawakami et al.
Tsai et al, "Rapid Separation and Quantification of Mixed Microorganisms by Filtration . . . ", 1986, Proceedings of the Soc., pp. 74-80.
Hirose, deceased Atsumi
Kataoka Takuji
Seto Susumu
Wang David
Nihon Millipore Kabushiki Kaisha
Rollins John W.
Ware Deborah K.
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