Method for counting megakaryocytes

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Concentration – separation – or purification of micro-organisms

Reexamination Certificate

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C435S029000, C435S288700, C422S082070

Reexamination Certificate

active

07569373

ABSTRACT:
A method for counting megakaryocytes in a specimen is described. In the method, first, erythrocytes in the specimen are lysed and nucleic acid in the megakaryocytes is stained with a fluorescent dye, and thereby, a measurement sample is prepared. Next, the cells in the measurement sample are irradiated with excited light so that the forward scattered light, the side scattered light and the fluorescence, which are emitted from the cells, are detected. Megakaryocytes are identified on the basis of the detected forward scattered light, the fluorescence and the side scattered light. Then, the identified megakaryocytes are counted.

REFERENCES:
patent: 2004/0009471 (2004-01-01), Cao
patent: 2005/0003471 (2005-01-01), Wang et al.
patent: 2005/0202400 (2005-09-01), Tsuji et al.
patent: 1 542 008 (2005-06-01), None
Aaron Tomer, et al “Flow Cytometric Analysis of Normal Human Megakaryocytes”, Blood, vol. 71, No. 5, May 1988, pp. 1244-1252.
Diamond et al, “Flow Cytometry in the Diagnosis and Classification of Malignant Lymphoma and Leukemia”, Cancer, American Cancer Society, vol. 50, No. 6, Sep. 15, 1982, pp. 1122-1135, XP008064994.
Petriz et al, “Relevance of Forward Scatter and Side Scatter in Aneuploidy Detection By Flow Cytometry”, Analytical Cellular Pathology, Elsevier Science, Amsterdam, NL, vol. 10, No. 3, May 1996, pp. 243-252, XP008064996.

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