Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1991-02-21
1995-02-14
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
435 29, 435808, 436 52, 436800, 2504581, 2504612, 359196, 359197, 359232, 356213, C12M 134, C12M 100, G01N 2100
Patent
active
053895444
ABSTRACT:
A method for counting living cells of microbes in a fluid sample continuously while flowing the sample using an apparatus which comprises a system for supplying at a predetermined rate to the flow line of the sample a reagent, such as, a derivative of fluorescein, capable of reacting with one or more substances intrinsic of the living cell, such as enzyme, to form an accumulative fluorescent product within the living cells; a reactor inserted in the flow line of the sample and being provided for the reaction of the reagent with the cell-intrinsic substance in the living cells; a photometric detection system arranged subsequent to the reactor for detecting fluorescence emitted as individual luminous point from the fluorescent product in each of the living cells floating in the flowing sample upon irradiation of the fluorescent product by an exciting ray; and an electronic unit including a pulse counter for counting electric pulses produced from each fluorescence from the luminous point.
REFERENCES:
patent: 3657537 (1972-04-01), Wheeless, Jr. et al.
patent: 4698308 (1987-10-01), Ikeda
Shechter, et al. FEBS Letters. vol. 139 No. 1 pp. 121-124 (1982).
Haugland, R. Handbook of Fluorescent Probes and Research Chemicals. Molecular Probes, Eugene, Oreg. (1989) pp. 105-113.
"Review of Scientific Instruments", J. A. Steinkamp, vol. 55, No. 9 pp. 1375-1400.
Doi Takashi
Matsumoto Kazunori
Onishi Takashi
Sugata Kiyoshi
Ueda Ryohei
Mitsubishi Jukogyo Kabushiki Kaisha
Williams Jane A.
Wityshyn Michael G.
LandOfFree
Method for counting living cells of microbes and apparatus there does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for counting living cells of microbes and apparatus there, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for counting living cells of microbes and apparatus there will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-287353