Data processing: measuring – calibrating – or testing – Measurement system in a specific environment – Biological or biochemical
Patent
1997-09-12
2000-03-07
Hoff, Marc S.
Data processing: measuring, calibrating, or testing
Measurement system in a specific environment
Biological or biochemical
702 20, 702 27, 39550032, G06G 748
Patent
active
060352582
ABSTRACT:
The operator downloads, from a data file into the random access memory of a personal computer, integrated optical density and profile area data for a plurality of nuclei and partial nuclei in a Feulgen-stained histologic tissue section; the thickness of the section; and the integrated optical density of an intact diploid nucleus. The operator then operates a computer program which plots the data curve together with a reference line and a reference curve. The data curve is scaled and the reference curve is recalculated and redrawn through a number of iterations until the operator is satisfied that the scaled data curve terminates on the reference line, and the scaled data curve is as nearly congruent as possible with the latest reference curve. The program calculates and displays the corrected DNA index on a video screen, the contents of which the operator may print on a printer.
REFERENCES:
patent: 5235522 (1993-08-01), Bacus
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McCready RW and Papadimitrious JM. An analysis of DNA cytomorphometry on tissue sections in a rat liver model. Analytical and Quantitative Cytology 5:117-123, 1983.
Bins M, Takens F. A method to estimate the DNA content of whole nuclei from measurements made on thin tissue sections. Cytometry 6:234-237, 1985.
Rigaut JP, PersozA. The `corpuscle` stereological problem--re-evaluation using slab fragment volumes and applications to the correction of DNA histograms from sections of spherical nuclei. Journal of Microscopy 156: 371-382, 1989.
Haroske G, MeyerW, DimmerV,et al. Feasibility and limitations of a cytometric DNA ploidy analysis procedure in tissue sections. Zentralblatt fur Pathologie 139 : 407-417, 1993.
Freed JA. Possibility of correcting image cytometric nuclear DNA (ploidy) measurements in tissue sections. Insights from computed corpuscle sectioning and the reference curve method. Analytical and Quantitative Cytology and Histology 19:376-386, 1997.
Hoff Marc S.
Vo Hien
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