Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1995-06-07
1998-05-26
Ketter, James
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
4242091, C12N 702, A61K 39145
Patent
active
057563411
ABSTRACT:
A method for producing Influenza and other viruses and vaccines derived therefrom utilizes serum-free cultured vertebrate cells or vertebrate biomass aggregates to both eliminate the necessity to use costly methods requiring whole chicken embryos and, optionally, to provide proteases suitable for the activation of a wide variety of viruses. In one aspect, the method comprises the periodic or continuous removal of "treatment portions" of virus-containing culture medium into an "augmentation loop" for treatment with a broad range of substances, such as proteases that augment the activation of the virus. Use of the loop allows utilization of such substances at high concentrations while eliminating their cell toxic effects. Another aspect of the invention provides for the alteration of cleavage sites in virus proteins to thereby render them more susceptible to activation in culture. Thus, the method provides for the high yield production of many viruses that can be easily scaled up to continuous large scale production volumes and for resultant vaccines which are free of egg proteins and are much more economical to produce.
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Barrett Noel
Dorner Friedrich
Kistner Otfried
Mundt Wolfgang
Brusca John S.
Immuno AG
Ketter James
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