Method for controlling gene expression

Details Method for controlling gene expression Method for controlling gene expression

1995-06-01

1997-04-22

Ketter, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4353201, 530350, 536 237, C12P 2102, C07K 14245, C12N 1531

Patent

active

056228406

ABSTRACT:
A lactose repressor protein wherein at least one amino acid at the position of 94, 241, 265 or 300 in the wild lactose repressor is replaced with an amino acid other than that of the wild lactose repressor, and the use thereof.
The present mutant lactose repressor represses the expression of a desired gene at 30.degree. C. or lower temperature, and induces the expression of a desired gene at 37.degree. C. or higher temperature, and therefor can control the expression by change of a culture temperature without using an expensive inducer such as IPTG.

REFERENCES:
Ulrich Deuschle et al., Proc. Natl. Acad. Sci., vol. 86, pp. 5400-5405, 1989.
Mickey C.T. Hu et al., Mol.Cell. Biol., vol. 10, pp. 6141-6151, 1990.
J.H. Miller, A Short Course in Bacterial Genetics, Laboratory Manual, pp. 131-134, Cold Spring Harbor Laboratory, 1992.
Huynh T.V. et al, DNA Cloning, vol. 1, IRL Press Limit. Oxford, England pp. 49-78 (1985).
Experiments in Molecular Genetics, pp. 352-355, Cold Spring Harbor Laboratory, 1972.

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