Method for controlling biooxidation reactions

Details Method for controlling biooxidation reactions Method for controlling biooxidation reactions

2007-09-18

2007-09-18

Lilling, Herbert J. (Department: 1657)

Chemistry: molecular biology and microbiology

Condition responsive control process

C435S134000, C435S136000, C435S142000

Reexamination Certificate

active

10756240

ABSTRACT:
Biooxidation reactions can be controlled by a method which comprises the steps of: (1) independently adding a substrate and a co-substrate at predetermined rates to a bioxidation reaction mixture comprised of a biocatalyst; (2) measuring the oxygen consumption rate and carbon dioxide evolution rate from the reaction mixture; (3) determining the instantaneous rates of substrate and cosubstrate consumption by solving simultaneous equations relating carbon dioxide evolution rate and oxygen consumption rate to the substrate oxidation stoichiometry, the cosubstrate combustion stoichiometry, and optionally the biomass formation stoichiometry; (4) simultaneously adjusting the substrate and cosubstrate addition rates to the rates of substrate oxidation and cosubstrate consumption in order to maximize the rate of product formation while simultaneously minimizing the rate of cosubstrate usage. The method provides a rapid means of controlling fed-batch biooxidation reactions which can employ in-line techniques and is broadly applicable for diverse oxidation reactions.

REFERENCES:
patent: 5254466 (1993-10-01), Picataggio et al.
patent: 5962285 (1999-10-01), Anderson et al.
patent: 6569670 (2003-05-01), Anderson et al.
U.S. Appl. No. 09/663,963, filed Sep. 19, 2000, Anderson et al.
Dixon, “Viable but Nonculturable”, ASM NEWS, vol. 64, No. 7, (1998), pp. 372-373.
Rehm, et al., “Mechanisms and Occurrence of Microbial Oxidation of Long-Chain Alkanes”, Adv. Biochem Eng., vol. 9, (1981), pp. 175-215.
Picataggio, et al., “Metabolic Engineering ofCandida tropicalisFor The Production Of Long-Chain Dicarboxylic Acids”, BIO/TECHNOLOGY, vol. 10, (Aug. 1992), pp. 894-898.
Gallo, et al., “Alkane Oxidation InCandida tropicalis”, Biochimica et Biophys. Acta., vol. 296, (1973), pp. 624-638.
Kemp, et al., “Inducible long chain alcohol oxidase from alkane-grownCandida tropicalis”, Applied Microbiology and Biotechnology, vol. 29, Springer-Verlag, (1988), pp. 370-374.
Scheller, et al., “Oxygenation Cascade in Conversion of n-Alkanes to α,ω-Dioic Acids Catalyzed by Cytochrome P450 52A3”, The Journal of Biological Chemistry, vol. 273, No. 49, (1998), pp. 32528-32534.
Eschenfeldt, et al., “Transformation of Fatty Acids Catalyzed by Cytochrome P450 Monooxygenase Enzymes ofCandida tropicals”, Applied and Environmental Microbiology, vol. 69, No. 10, (Oct. 2003), pp. 5992-5999.
Wackett, et al., Biocatalysis and Biodegradation, Chapter 2, ASM Press, (2001), pp. 15-18.
Stryer, Biochemistry—Danger lurks in the reduction of oxygen, 4th Edition, W. H. Freemen and Co., (1999), p. 540.
Hosobuchi, et al., “Application of Computer to Monitoring and Control of Fermentation Process: Microbial Conversion of ML-236B Na to Pravastatin”, Biotechnology and Bioengineering, vol. 42, No. 7, John Wiley & Sons, Inc., (Sep. 20, 1993), pp. 815-820.
Lee, et al., “Control of fed-batch fermentations”, Biotechnology Advances, vol. 17, Elsevier Science Inc., (1999), pp. 29-48.
Coppella, et al., “Low-Cost Computer-Coupled Fermentor Off-Gas Analysis via Quadrupole Mass Spectrometer”, Biotechnology and Bioengineering, vol. 29, John Wiley & Sons, Inc., (Apr. 1987), pp. 679-689.

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