Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-20
1999-11-30
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C12P 1934, C12N 1500
Patent
active
059940581
ABSTRACT:
A new contiguous genome sequencing method is described which allows the contiguous sequencing of a very long DNA without need to be subcloned. It uses the basic PCR technique but circumvents the usual need of this technique for the knowledge two primers for contiguous sequencing, enabling the knowledge of only one primer sufficient. The present invention makes it possible to PCR amplify a DNA adjacent to a known sequence with which one primer can be made without the knowledge of the second primer binding site present in the unknown sequence. The present invention could thus be used to contiguously sequence a very long DNA such as that contained in a YAC clone or a cosmid clone, without the need for subcloning smaller fragments, using the standard PCR technique. It can also be used to sequence a whole chromosome or genome without any need to subclone it.
REFERENCES:
patent: 5104792 (1992-04-01), Silver et al.
patent: 5106727 (1992-04-01), Hartley et al.
patent: 5108892 (1992-04-01), Burke et al.
patent: 5407799 (1995-04-01), Studier
patent: 5487985 (1996-01-01), McClelland et al.
patent: 5487993 (1996-01-01), Herrnstadt et al.
patent: 5508169 (1996-04-01), Deugau
patent: 5731171 (1998-03-01), Bohlander
DNA Sequence--Journal DNA Sequencing and Mapping, vol. 2, issued 1992, P. Verhasselt et al., *DNA sequencing by a subcloning-walking strategy using a specific and semi-random primer in the polymerase chain reaction*, pp. 281-287.
Sarkar et al., DNA and Cell Biology 12 (7): 611-615 (1993).
Loh et al., Science 243: 217-220 (1988).
Volinia et al., CABIOS 5: 33-40 (1989).
Liu et al., Genomics 25: 674-681 (1995).
Kotler et al., PNAS 90: 4241-4245 (1993).
Caetano-Anolles et al., Mol. Gen. Genet. 235:157-165 (1992).
Nevinsky et al., Biochemistry 29:1200-1207 (1990).
Studier, F W., PNAS 86: 6971-6921 (1989).
Parker et al. Nucleic Acids Research 19(11):3055-3060.
Genome International Corporation
Jones W. Gary
Whisenant Ethan
LandOfFree
Method for contiguous genome sequencing does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for contiguous genome sequencing, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for contiguous genome sequencing will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1669922