Method for constructing an oligonucleotide concatamer library by

Details Method for constructing an oligonucleotide concatamer library by Method for constructing an oligonucleotide concatamer library by

1995-05-09

1997-07-15

Guzo, David

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 914, 435 9141, C12P 1934, C12N 1564, C12N 1566

Patent

active

056482450

ABSTRACT:
An in vitro method for constructing an oligonucleotide concatamer library is revealed. The method has four steps. The first step involves the generation of a primer-bridged circular template oligonucleotide. In the second step, the primer-bridged circular template is used to generate a single-stranded oligonucleotide concatamer by rolling circle replication. In the third step, the single-stranded oligonucleotide concatamer is converted to a double-stranded oligonucleotide concatamer. Finally, the double-stranded oligonucleotide concatamer is cloned or used directly in in vitro assays which allow molecules of interest to be isolated or amplified.

REFERENCES:
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Vergnaud, et al., "The Use of Synthetic Tandem Repeats to Isolate New Vntr Loci: Cloning of a Human Hypermutable Sequence", Genomics 11, 1991, pp. 135-144.

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