Method for conducting capillary zone electrophoresis

Details Method for conducting capillary zone electrophoresis Method for conducting capillary zone electrophoresis

2005-08-16

2005-08-16

Diamond, Alan (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S453000, C204S601000

Reexamination Certificate

active

06929729

ABSTRACT:
A method for conducting capillary zone electrophoresis in which a lubricating detergent is added to help prevent sample buildup on the inner walls of the capillary. In a preferred embodiment the lubricating detergent is sodium dodecylsulfate (SDS) in a concentration of about 3 mM added to a protein sample before the sample is introduced into one end of a capillary. The SDS may be added to the buffer, instead of the sample, before electrophoresis.

REFERENCES:
patent: 4997536 (1991-03-01), Ohms et al.
patent: 5124020 (1992-06-01), Wang
patent: 5459272 (1995-10-01), Novotny et al.
patent: 5792330 (1998-08-01), Petersen et al.
patent: 8-114575 (1996-05-01), None
Kenndler et al, Journal of Chromatography, 545, (1991), pp. 397-402.
Hu et al, Journal of Chromatography A, 717, (1995), pp. 33-39.

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