Method for conducting a polymerase chain reaction using an impro

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 911, 435 912, 435183, 436 94, 436800, 436805, 436905, 536 231, 536 2432, 536 2433, 536 253, 428917, 935 77, 935 78, C12Q 168, C12P 1934, C12N 1500

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active

056100175

ABSTRACT:
This invention relates to a new electrochemiluminescent (ECL) label for oligonucleotides using phosphoramidite chemistry.

REFERENCES:
patent: 4725677 (1988-02-01), Koster et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 5061445 (1991-10-01), Zoski et al.
patent: 5135717 (1992-08-01), Rezoni et al.
Beaucage, S.L. and Caruthers, M.H., "Deoxynucleoside phosphoramidites, a new class of key intermediates for deoxypolynucleotide synthesis", Tetrahedron Lett. 22, 1859-62 (1982).
Gray, P.W. and Goeddel, D.V., "Structure of the human immune interferon gene", Nature 298, 859-863 (1982).
Shibata, D. K., Arnheim, N.B., and Martin, J.W., "Detection of human papilloma virus in paraffin-embedded tissue using the polymerase chain reaction", J. Exp. Med. 167, 225-30 (1988).
Yee C., Krishnan-Hewlett, I., Baker C. C., Schlegel, R., and Howley, P. M., "Presence and Expression of Human Papillomavirus sequences in human cervical carcinoma cell lines", Am. J. Pathol. 119, 361-6 (1985).
Mullis, K.B., and Falcoona, F. A., "Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction", Methods Enzymol 155, 333-50 (1987).
Barone, A. D., Tang, J-Y, and Caruthers, M. H., "In situ activation of bis-dialkylaminophosphines -a new method for synthesizing deoxyoligonucleotides on polymer supports", Nucleic Acids Res. 12, 4051-61 (1984).
Updyke, T. V. and Nicolson, G. L., "Immunoaffinity isolation of membrane antigens with biotinylated monoclonal antibodies and streptavidinagarose", Methods Enzymol. 121, 717-25 (1986).
Cardullo, R. A., Agrawal, S., Flores, C., Zamecnik, D. C., and Wolf, D. E., "Detection of nucleic and hybridization by nonradiative fluorescence resonance energy transfer", Proc. Natl. Acad. Sci. 85, 8790-4 (1988).
Ou, C-Y, Kwok, S., Mitchell, S. W. Mack, D. H., Sninsky, J. J., Kreds, J. W., Feorino, P., Warfield, D., and Schochetman, G., "DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells", Science 239, 295-97 (1988).
Beaucage, S. L., "A Simple and Efficient Preparation of Deoxynucleoside Phosphoramidites in Situ," Pergamon Press Ltd., 375-378 (1984).
Bannworth et al., "A Simple Specific Labelling for oligonucleotides by Barthophenanthroline, RuII complexes as Nonradioactive Label Molecules", Tetrahedron Letters, vol. 30, No. 12, pp. 1513-1516, 1989.
Erhan and Greller, "Do Immunoglobulins have proteolytic activity" Nature, vol. 251, 353-355 (1974).
Y. Weinstein, et al. "Cyclic GMP Stimulates lympphocyte nucleic acid synthesis" Nature, vol. 251, 352-353, (1974).
Blackburn et al., "Electrochemiluminescence Detection for Development of Immunoassays and DNA Probe Assays for Clinical Diagnostics", Clinical Chemistry 37, 1534-39 (1991).
Kenten, J. H., Gudibande S., Kink, J., Willey, J., Curfman, B., Major, E. O., Massey, R. J., "Improved Electrochemiluminescent Label for DNA Probe Assays: Rapid Quantitative Assays of HIV-1 Polymerase Chain Reaction Products", Clinical Chemistry 38, 873-879 (1992).
Kenten, J. H., Casadei, J., Link, J., Lupold, S., Willey, J., Powell, M., Rees,A., Massey, R., "Rapid Electrochemiluminescence Assays of Polymerase Chain Reaction Products", Clinical Chemistry 37, 1626-32 (1991).
Sommer et al., "Minimal Homology Requirements for PCR Primers" Nucleic Acids Research, vol. 17, No. 16 p. 6749 (1989).
Rodriguez et al., "Electrochemical Studies of the Interation of Metal Chelates with DNA. 4. Voltammetric and Electrogenerated Chemiluminescent Studies of the Interation of Tris (2,2'-bipyridine)osminum (II) with DNA," Anal Chem. vol. 62 (1990) pp. 2658-5662.

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