Method for conditioning and cryopreserving cells

Details Method for conditioning and cryopreserving cells Method for conditioning and cryopreserving cells

1998-10-07

2000-10-31

Naff, David M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

424 931, 435243, 435260, 435261, 435325, 435404, A01N 6300, A01N 6500, C12N 100, C12N 102, C12N 500

Patent

active

061401234

ABSTRACT:
A method is provided for preconditioning and cryopreservation of cells harvested from a donor. Cells are suspended in a cell conditioning and cryopreservation medium containing a cryopreservative such as Dimethyl Sulfoxide (DMSO) and the suspension is incubated for a period of at least ten minutes and the cells are frozen. The medium includes adenosine, a calcium channel blocker, and a cell nutrient matrix comprising a sufficient amount of cell nutrients to sustain the metabolic needs of the cells during incubation without producing detectable levels of lactate or substantially depleting the nutrient substrates to maintain viability of the harvested cells. In some embodiments, a cryopreservative is added step-wise before the cell suspension is frozen and removed step-wise after the cell suspension is thawed.

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