Method for collecting and preserving semen

Details Method for collecting and preserving semen Method for collecting and preserving semen

2005-03-08

2005-03-08

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Maintaining blood or sperm in a physiologically active state...

C435S374000, C424S561000

Reexamination Certificate

active

06864046

ABSTRACT:
A method for collecting and preserving semen of various animals including humans, canines, porcines, bovines, ovines and others involves collecting the semen into a collection vessel where the collection vessel is provided with an extender solution for the semen prior to its collection. Moreover, the extended is preferably maintained at a temperature close to normal body temperature of the species being collected over the time period of its collection. The extender is chosen to buffer the pH of the semen sample and to be isotonic with the semen. The volume of the extender in the collection vessel is preferably chosen such that the semen volume is initially diluted with twice its volume extender solution and some period thereafter the extended semen sample is diluted again at the same ratio. Collection into warmed extender media lessened the cold and pH shock to the spermatozoa, as shown by improved semen parameters. The extender solution is preferably rich in calcium ion. A collection vessel resembling an inverted Y is used for collecting distinct semen samples for comparative study.

REFERENCES:
patent: 3472735 (1969-10-01), Yoshimasa
patent: 3973003 (1976-08-01), Colas
patent: 4007087 (1977-02-01), Ericsson
patent: 4329337 (1982-05-01), Sexton
patent: 4965186 (1990-10-01), Grischenko
patent: 5494800 (1996-02-01), Smith, III
patent: 5495719 (1996-03-01), Gray, Jr.
patent: 5849473 (1998-12-01), Cabrera
patent: 5863715 (1999-01-01), Rajotte
patent: 5961503 (1999-10-01), Simmet et al.
patent: 5983661 (1999-11-01), Wiesman
Bearden, J.H.; Fuquay, J.W., “Applied Animal Reproduction,” Applied Animal Reproduction, 5th ed., Prentice-Hall (Upper Saddle River, New Jersey), p. 183-186.
Brinsko, S.P.; Varner, D.D., “Artifical Insemination and Preservation of Semen,” Stallion Management, Veterinary Clinics of North America: Equine Practice, vol. 8 (No. 1), p. 205-218, (Apr. 1, 1992).
Grootegoed, J.A.; Den Boer, P.J., “Energy Metabolism of Spermatids: A Review,” Cellular and Molecular Events in Spermiogenesis, Cambridge University Press (Cambridge, UK), p. 193-217, (Jun. 1, 1987).
Prins, G.S.; Weidel, L., “A Comparative Study of Buffer Systems and Cryoprotectants for Human Spermatozoa,” Fertility and Sterility, The American Fertility Society (USA), vol. 46 (No. 1), p. 147-149, (Jul. 1, 1986).
Prien, S.D., “A Comparative Study of Calcium Utilization in Human and Porcine Spermatozoa,” Animal Science, p. 28-150, (Aug. 1, 1991).
Keel, B.A.; Webster, B.W., “Laboratory Diagnosis and Treatment of Fertility,” CRC Handbook, CRC Press (USA), p. 197-199.
Tao, J.; Du, J.; Kleinhans, F.W.; Critser, E.S.; Mazur, P.C.; Critser, J.K., “The Effect of Collection Temperature, Cooling Rate and Warming Rate on Chilling Injury and Cryopreservation of Mouse Spermatozoa,” Journals of Reproduction & Fertility, p. 231-236, (Jun. 1, 1995).
Wright, P.J.; Parry, B.W., “Cytology of the Canine Reproductive System,” Clinical Pathology: Part II, Veterinary Clinics of North America: Small Animal Practice, vol. 19 (No. 5), p. 851-874, (Sep. 1, 1989).
Seager, S.W.J.; Platz, C.C., “Collection and Evaluation of Canine Semen,” Symposium on Reproductive Problems, Veterinary Clinics of America, vol. 7 (No. 4), p. 765-773, (Nov. 1, 1977).
Sherman, J.K., “Cryopreservation of Human Semen,” Techniques of Human Andrology, Elsevier/North Holland Biomedical Press, p. 400-420, (Jun. 1, 1977).

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