Method for coarse purification of cell digests from...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...

Reexamination Certificate

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C435S270000, C435S006120, C536S023100

Reexamination Certificate

active

10504258

ABSTRACT:
The present invention relates to the coarse clarification of lysed cell material from microorganisms and particularly to a method of obtaining nucleic acids. In certain embodiments, the present invention provides a method of coarsely clarifying cell lysate from microorganisms that comprises: lysing the microorganisms under the normal atmospheric pressure to form a flocculent precipitate in the lysate, compressing the flocculent precipitate in the lysate by reducing or increasing the pressure of the atmosphere surrounding the lysate in relation to the normal atmospheric pressure to form a compressed flocculent phase and a liquid phase, and separating the two phases.

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patent: 2006/0166331 (2006-07-01), Au-Yeung et al.
patent: 0 875 271 (1998-11-01), None
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Heilig, J.S., Eberling, K.L., and Brent, R. 1998. Preparation of Crude Lysate by Alkaline Lysis in Large-scale Preparation of Plasmid DNA. Unit 1.7.
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Atmospheric puressure article from Wikipedia, retrieved from http://en.wikipedia.org/wiki/Atmospheric—pressure.
Itoh M et al. “Automated filtration-based high throughput plasmid preparation system”, Genome Research, 1999, vol. 9, p. 463-470.
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