Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2008-07-29
2008-07-29
Kim, Young J. (Department: 1637)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S006120
Reexamination Certificate
active
10477907
ABSTRACT:
The present invention relates to a non-PCR (polymerase chain reaction) process, particularly a transcription-based amplification method, for amplifying and cloning sequences containing a variable domain sequence such as an immunoglobulin variable domain sequence from the immunological gene repertoire. The present invention comtemplates the expression of antibody library in either in an in vivo expression vector or in an in vitro transcription/translation system. Isolation of a gene coding for a receptor having the ability to bind a preselected ligand and receptors produced by the gene isolated by the method is also contemplated.
REFERENCES:
patent: 5554516 (1996-09-01), Kacian et al.
patent: 5627052 (1997-05-01), Schrader
patent: 5667988 (1997-09-01), Barbas et al.
patent: 5965408 (1999-10-01), Short
Kim Young J.
San Diego Antibody
Townsend and Townsend and Crew
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