Method for cloning the NSPHI restriction-modification system in

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

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4353201, 4352523, 536 232, C12N 922, C12N 1555

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active

061300780

ABSTRACT:
The present invention relates to recombinant DNA molecules encoding NspHI restriction endonuclease and methylase and to method to use premodified E. coli K strain RR1 (.lambda.DE3) for overexpression of NspHI restriction endonuclease.

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