Method for cloning secreted proteins

Details Method for cloning secreted proteins Method for cloning secreted proteins

1998-07-24

2000-05-23

Schwartzman, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 536 231, C12Q 168, C12P 1934, C12N 1511, C07H 2100

Patent

active

060664606

ABSTRACT:
The invention provides a method for isolating nucleic acids encoding proteins comprising a signal peptide, e.g., a secreted protein. The method of the invention comprises isolating an RNA molecule associated with an endoplasmic reticulumn membrane preparation under conditions wherein the RNA is at least partially translated. The invention also provides a library of nucleic acids encoding proteins comprising a signal peptide, individual nucleic acids isolated according to the method of the invention, peptides encoded thereby, pharmaceutical compositions comprising such and kits for performing the method of the invention.

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