Method for cloning restriction modification system

Details Method for cloning restriction modification system Method for cloning restriction modification system

1992-08-11

1994-06-14

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435193, 435199, C12N 1555, C12N 922

Patent

active

053209575

ABSTRACT:
A two-step method for cloning restriction modification systems is provided which comprises introducing the modification or methylase gene into a host cell and expressing the gene therein to protect the host cell followed by introducing the restriction or endonuclease gene into the host whereby the restriction gene is expressed at lower levels relative to the modification gene. Related methods for producing restriction enzymes is also disclosed as well as clones containing the BamI and DdeI restriction genes.

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