Method for cloning and producing the PshAI restriction endonucle

Details Method for cloning and producing the PshAI restriction endonucle Method for cloning and producing the PshAI restriction endonucle

1996-03-06

1998-10-20

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4353201, 43525233, 536 232, C12N 922, C12N 1555

Patent

active

058245294

ABSTRACT:
The present invention is directed to a method for cloning and producing the PshAI restriction endonuclease by (1) introducing the restriction endonuclease gene from Plesiomonas shigelloides into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the PshAI restriction endonuclease activity, and (3) purifying the PshAI restriction endonuclease from the fermented host which contains the vector encoding and expressing the PshAI restriction endonuclease activity.

REFERENCES:
patent: Re35248 (1996-05-01), Benner et al.
patent: 5200333 (1993-04-01), Wilson
Kosykh, et al., Molec. Gen. Genet., 178:717-719 (1980).
Mann, et al., Gene, 3:97-112 (1978).
Walder, et al., Proc. Nat. Acad. Sci., 78:1503-1507 (1981).
Bougueleret, et al., Nucl. Acid Res., 12:3659-3676 (1984).
Gingeras and Brooks, Proc. Natl. Acad. Sci. USA 80:402-406 (1983).
Theriault and Roy, Gene, 19:355-359 (1982).
Blumenthal, et al., J. Bacteriol., 164:501-509 (1985).
Kiss, et al., Nucl. Acid Res., 13:6403-6421 (1985).
Szomolanyi, et al, Gene, 10:219-225 (1980).
Janulaitis, et al., Gene, 20:197-204 (1982).
Kiss and Baldauf, Gene, 21:111-119 (1983).
Walder, et al., (J. Biol. Chem., 258:1235-1241 (1983).
Piekarowicz, et al., Nucleic Acid Res. 19:1831-1835 (1991).
Piekarowicz, et al., J. Bacteriology, 173:150-155 (1991).
Fomenkov, et al., Nucleic Acids Res. 22:2399-2403 (1994).
Lunnen, et al., Gene, 74:25-32 (1988).
Raleigh and Wilson, Proc. Natl. Acad. Sci, USA, 83:9070-9074 (1986).
Heitman and Model, J. Bact., 196:3243-3250 (1987).
Raleigh, et al., Genetics, 122:279-296 (1989).
Waite-Rees, et al., J. Bacteriology, 173:5207-5219 (1991).
Wilson, Methods in Enzymology, 216:259-279 (1992).
Ochman, et al., Genetics, 120:621 (1988).
Triglia, et al., Nucl. Acids Res. 16:8186 (1988).
Silver and Keerikatte, J. Cell Biochem., (Suppl.) 13E:306, Abstract No. WH239 (1989).
Brooks, et al. Nucleic Acid Res. 17:979-997 (1989).
Fuller, Gene, 19:43-54 (1982).
Shimatake and Rosenberg, Nature, 292:128 (1981).
Shine & Dalgarno, Proc. Natl. Acad. Sci. USA, 71:1342-1346 (1974).
Ikemura, J. Mol. Biol., 151:389-409 (1981).
Miyahara, M., et. al. (1990) Gene 87, 119-122.
Lunnen, K. D, et. al. (1988) Gene 74, 25-32.

Affiliated with

Also associated with

Rating

Method for cloning and producing the PshAI restriction endonucle does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for cloning and producing the PshAI restriction endonucle, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for cloning and producing the PshAI restriction endonucle will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-243378