Method for cloning and producing the NspI restriction endonuclea

Details Method for cloning and producing the NspI restriction endonuclea Method for cloning and producing the NspI restriction endonuclea

1998-08-18

2000-02-22

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 4353201, 536 232, C12N 922, C12N 1555

Patent

active

06027929&

ABSTRACT:
The present invention relates to clones which express the recombinant NspI restriction endonuclease using a NspI methylase premodified E. coli K strain RR1 (.lambda.DE3) and to methods for producing and purifying said enzyme.

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