Method for cloning and producing the NCO I restriction endonucle

Details Method for cloning and producing the NCO I restriction endonucle Method for cloning and producing the NCO I restriction endonucle

1990-11-02

1993-04-13

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435143, 43525233, 4353201, 536 232, C12N 922, C12N 1555

Patent

active

052022480

ABSTRACT:
The present invention is directed to a method for cloning and producing the NcoI restriction endonuclease by 1) introducing the restriction endonuclease gene from N. corallina into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the NcoI restriction endonuclease activity, and 3) purifying the NcoI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the NcoI restriction endonuclease activity.

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