Method for cloning and producing the DraIII restriction endonucl

Details Method for cloning and producing the DraIII restriction endonucl Method for cloning and producing the DraIII restriction endonucl

1999-01-22

2000-04-11

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352523, 43525233, 4353201, 435478, 536 232, C12N 922, C12N 1555

Patent

active

060487194

ABSTRACT:
The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.

REFERENCES:
patent: 4863858 (1989-09-01), Grosskopf
patent: 5200333 (1993-04-01), Wilson
patent: 5320957 (1994-06-01), Brooks et al.
patent: 5492823 (1996-02-01), Xu et al.
Roberts et al, Nucl. Acids Res. 26:338-350 (1998).
Kosykh et al., Mol. Gen. Genet. 178:717-719 (1980).
Mann et al., Gene 3:97-112 (1978).
Walder et al., Proc. Nat. Acad. Sci. 78:1503-1507 (1981).
Bouqueleret et al., Nucl. Acids Res. 12:3659-3676 (1984).
Gingeras et al., Proc. Natl. Acad. Sci. USA 80:402-406 (1983).
Theriault et al., Gene 19:355-359 (1982).
Blumenthal et al., J. Bacteriol. 164:501-509 (1985).
Looney, et al., Gene, 80:193-208 (1989).
Kiss et al., Nucl. Acids. Res. 13:6403-6421 (1985).
Szomolanyi et al., Gene 10:219-225 (1980).
Janulaitis et al., Gene 20:197-204 (1982).
Kiss et al., Gene 21:111-119 (1983).
Walder et al., J. Biol. Chem. 258:1235-1241 (1983).
Fomenkov et al., Nucl. Acids Res. 22:2399-2403 (1994).
Piekarowicz, et al., Nucl. Acids Res., 19:1831-1835 (1991).
Piekarowicz, et al., J. Bacteriology 173:150-155 (1991).
Lunnen, et al., Gene 74:25-32 (1988).
Raleigh & Wilson, Proc. Natl. Acad. Sci. USA, 83:9070-9074 (1986).
Heitman and Model, J. Bacteriology, 169:3243-3250 (1987).
Raleigh, et al., Genetics, 122-279-296 (1989).
Waite-Rees, et al., J. Bacteriology, 173:5207-5219 (1991).
Ochman, et al., Genetics, 120:621 (1988).
Triglia, Nucl. Acids Res., 16:8186 (1988).
Silver and Keerikatte, J. Cell. Biochem (Suppl.) 13E:306 Abstract No. WH239 (1989).
Ives, et al., J. Bacteriology, 177:6313 (1995).
Matsudaira, J. Biol. Chem., 262:10035-10038 (1987).

Affiliated with

Also associated with

Rating

Method for cloning and producing the DraIII restriction endonucl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for cloning and producing the DraIII restriction endonucl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for cloning and producing the DraIII restriction endonucl will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1175655