Method for cloning and producing the BglII restriction endonucle

Details Method for cloning and producing the BglII restriction endonucle Method for cloning and producing the BglII restriction endonucle

1993-04-09

1995-07-18

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Transferase other than ribonuclease

43525233, 4353201, 435193, 536 232, C12N 922, C12N 1555, C12N 121, C12N 1563

Patent

active

054340683

ABSTRACT:
The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the BglII restriction endonuclease activity; and 3) purifying the BglII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the BglII restriction endonuclease.

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