Method for cloning and expression of Tth111II restriction...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S320100, C435S252300, C536S023200

Reexamination Certificate

active

06919194

ABSTRACT:
The present invention relates to recombinant DNA which encodes the Tth111II restriction endonuclease-methylase fusion protein (Tth111IIRM), expression of Tth111II restriction endonuclease-methylase fusion protein inE. colicells containing the recombinant DNA, and purification of Tth111II endonuclease-methylase fusion protein to near homogeneity.

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