Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1995-02-16
1999-11-23
McElwain, Elizabeth F.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 9141, 435911, 435913, 435925, 435929, 435931, 435945, C12N 1500, C12N 114
Patent
active
059898704
ABSTRACT:
A method is described for the identification and cloning of promoters that express under a defined environmental condition, such as growth in glucose medium. Using this method, five Trichodermal promoters capable of the high expression of operably linked coding sequences are identified, one of which is the promoter for T. reesei tef1. Also provided are altered cbh1 promoters, altered so that glucose no longer represses expression from such promoter. The invention further provides vectors and hosts that utilize such promoters, and unique fungal enzyme compositions from such hosts.
REFERENCES:
patent: 4725535 (1988-02-01), Sonenshein et al.
patent: 5108918 (1992-04-01), Groenen et al.
patent: 5674707 (1997-10-01), Hintz et al.
patent: 5710021 (1998-01-01), Hintz et al.
Teeri, T. et al., "The Molecular Cloning of the Major Cellulase Gene from Trichoderma reesei," Bio/Technol.:696-699 (Oct. 1983).
Harkki, A. et al., "A Novel Fungal Expression System: Secretion Of Active Calf Chymosin From The Filamentous Fungus Trichoderma reesei," Bio/Technol. 7:596-603 (Jun. 1989).
Judelson, H.W. et al., "Highly Abundant and Stage-Specific mRNAs in the Obligate Pathogen Bremia lactucae," Molecular Plant-Microbe Interactions 3(4):225-232 (1990).
Turgeon, B.G. et al., "Development of a Fungal Transformation System Based on Selection of Sequences with Promoter Activity," Molec. and Cell. Biol. 7(9):3297-3305 (1987).
Curie, C. et al., "Cis and trans-acting elements involved in the activation of Arabidopsis thaliana A1 gene encoding the translation elongation factor EF-1.alpha.," Nucl. Acids Res. 19(6):13-5-1310 (1991).
Kim, D.W. et al., "Use of the human elongation factor 1.alpha. promoter as a versatile and efficient expression system," Gene 91:217-223 (1990).
Miyajima, A. et al., "Coordination of Levels of Elongation Factors Tu, Ts, and G, and Ribosomal Protein S1 in Escherichia coli," J. Biochem. 83:453-462 (1978).
Thiele, D. et al., "Elongation Factor 1.alpha. from Saccharomyces cerevisiae: Rapid Large-Scale Purification And Molecular Characterization," J. Biol. Chem. 260 (5):3084-3089 (1985).
Uetsuki, T. et al., "Isolation and Characterization of the Human Chromosomal Gene for Polypeptide Chain Elongation Factor-1.alpha.," J. Biol. Chem. 10:5791-5798 (1989).
Schirmaier, F. et al., "Identification of two genes coding for the translation elongation factor EF-1.alpha. of S. cerevisiae," EMBO J. 3(13):3311-3315 (1984).
Liboz, Thierry et al., "The four members of the gene family encoding the Arabidopsis thaliana translation elongator factor EF-1.alpha. are actively transcribed," Plant Mol. Biol. 14:107-110 (1989).
Auer, J. et al., "Organisation and Nucleotide Sequence of a Gene Cluster Comprising the Translation Elongation Factor 1.alpha. from Sulfolobus acidocaldarius," System. Appl. Microbiol. 14(1):14-22 (1990).
Ishiura, M. et al., "Simplified cosmid vectors for gene transfer to cultured mammalian cells: isolation of the gene for elongation factor 2 from the mouse," Gene 85:427-433 (1989).
Penttila, M.E. et al., "Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillus .beta.-glucosidase," Mol. Gen. Genet. 194(3):494-499 (1984).
von Melchner, H. et al., "Isolation of cellular promoters by using a retrovirus promoter trap," Proc. Natl. Acad. Sci. USA 87:3733-3737 (1990).
Neve et al., "Eukaryotic DNA fragments which act as promoters for a plasmid gene," Nature 277: 324-325 (1979).
Soldati, L. et al., "Effective gene expression in Pseudomonas aeruginosa under the control of the Escherichia coli consensus promoter," FEMS Microbiol. Letts. 42:163-167 (1987).
An, G. et al., "Plasmid Vehicles for Direct Cloning of Escherichia coli Promoters," J. Bacteriol. 140(2):400-407 (1979).
Band, L. et al., "Construction of a vector for cloning promoters in Bacillus subtilis," Gene 26 2(3):313-315 (1983).
Achen, M.G. et al., "Construction of plasmid vectors for the detection of streptococcal promoters," Gene 45 (1):45-49 (1986).
Goodey, A.R. et al., "The selection of promoters for the expression of heterologous genes in the yeast Saccharomyces cerevisiae," Mol. Gen. Genet. 204:505-511 (1986).
Pater, M.M. et al., "Thymidine Kinase of Herpes Virus as a Vehicle for the Isolation and Characterization of Unknown Mammalian Promoters and Enhancers," J. Mol. Appl. Genet. 2(4):363-371 (1984).
Slos, P. et al., "Isolation and Characterization of Chromosomal Promoters of Streptococcus salivarius subsp. thermophilus," Appl. Environ. Microbiol. 57(5):1333-1339 (1991).
Santangelo, G.M. et al., "Properties of Promoters Cloned Randomly from the Saccharomyces cerevisiae Genome," Molec. and Cell. Biol. 8(10):4217-4224 (1988).
Savochkina, L.P. et al., "Stability of Cloned Promoter-Containing Fragments," Mol. Gen. Genet. 189:142-147 (1983).
Ogawa, H. et al., "Molecular cloning of promoter-containing fragments from Bacillus stearothermophilus and their expression in Escherichia coli and Bacillus subtilis," FEMS Microbiol. Letts 24 (2-3):169-172 (1984).
Gatignol, A. et al., "Cloning of Saccharomyces cerevisiae promoters using a probe vector based on phleomycin resistance," Gene 91:35-41 (1990).
Goldfarb, D.S. et al., "Expression Probe Plasmids To Isolate And Analyze Bacillus Subtillus Regulatory Elements," Proc. Int. Symp. Genet. Ind. Microorganisms, pp. 120-124 (1982).
Kubota, M. et al., "Random screening of promoters from Escherichia coli and classification based on the promoter strength," Jpn. J. Genet. 66:399-409 (1991).
Flick, J.S. et al., "Two Systems of Glucose Represseion of the GAL1 Promoter in Saccharomyces cerevisiae," Mol. and Cell. Biol. 10(9):4757-4769 (1990).
Shoemaker, S.P. et al., "Cellulases: Diversity Amongst Improved Trichoderma Strains," In: Trends In The biology Of Fermentations For Fuels And Chemicals, Hollaender, Alexander (ed.), Plenum Press, New York, pp. 89-109 (1981).
Montenecourt, B.S. et al., "Preparation of Mutants of Trichoderma reesei with Enhanced Cellulase Production," Appl. and Environ. Microbiol. 34(6):777-782 (1977).
Takashima, S. et al., "Analysis of Cre1 binding sites in the Trichoderma resei cbhl upstream region," FEMS Microbiol. Letters 145:361-366 (1996).
Margret, D. and Lauquin, G.J.M. 1986. The Yeast SRP Gene: Positive Modulation by Glucose of its Transcriptional Expression, Biochemical and Biophysical Research Communications, 138(1):297-303.
Nakari et. al., Proceeding of the Second Tricel Symposium on Trichoderma reesei Cellulases and Other Hydrolases, Espoo, Finland, ed by P. Suominen et. al. Foundation for Biotechnical and Industrial Fermentation Research 8(1993):239-246.
Penttka et al, 1987, Gene 61:155-164.
Ilmen Marja Hannele
Nakari Tiina Hannele
Onnela Maija-Leena
Penttila Merja Elisa
McElwain Elizabeth F.
Rohm Enzyme Finland Oy
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