Method for cleaving chimeric protein using processing enzyme

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4352523, 435325, 530350, 536 234, C12P 2106, C07K 1400

Patent

active

058916717

ABSTRACT:
A chimeric protein is provided which comprises a target protein and has been designed so as to be easily cleaved by a processing enzyme, permitting a target protein to be efficiently recovered. The chimeric protein is represented by the following formula:

REFERENCES:
P.G. Seeboth et al., "In-Vitro Processing of Yeast .alpha.-Factor Leader Fusion Proteins Using a Soluble yscF (Kex2) Variant", Applied Microbiology Biotechnology (1991), vol. 35, pp. 771-776.
P.G. Seeboth et al., "In-Vitro Cleavage Of A Fusion Protein Bound To Cellulose Using the Soluble yscF.sub.s (Kex2) Variant", Applied Microbiology Biotechnology (1992), vol. 37, pp. 621-625.
Burgess et al. J. of Cell Biol. 11 : 2129-2138, 1990.
Lazar et al. Mol. Cell Biol. 8 : 1247-1252, 1988.
Tao et al. J. Immunol. 143(8) : 2595-2601, 1989.
Gillies et al. Human Antibodies & Hybridomas 1(1) : 47-54, 1990.
Wingender, E. et al. JBC. 264(8) : 4367-4373, 1989.
Friedman, TC. et al. Endocrinology 136(10):4462-4472, 1995.
Ledgerwood, EC et al. Biochem. J. 308 : 321-325, 1995.
Brennan, SO et al. FEBS letters. 347(1) : 80-84, 1994.
Diefenbach-Jagger, H. et al. Eur. J. Biochem, 229: 91-98, 1995.
Hendy, G.N. et al. JBC. 270(16): 9517-9525, 1995.
Jean et al. Biochem J. 292 : 891-900, 1993.
Koyama, N. et al. J. Biotechnol. 32(3) : 273-81, 1994.
Rouille, Y et al. JBC. 270(44) : 26488-26496, 1995.
Rothenberg, ME et al. JBC 270(17) : 10136-10146, 1995.
Drucker, DJ. Mol. Endocrinol 8(12) : 1646-55, 1994.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for cleaving chimeric protein using processing enzyme does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for cleaving chimeric protein using processing enzyme, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for cleaving chimeric protein using processing enzyme will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1370303

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.