Method for classifying leukocytes and a reagent used therefor

Details Method for classifying leukocytes and a reagent used therefor Method for classifying leukocytes and a reagent used therefor

1992-10-21

1995-02-14

Bhat, Nina

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 17, 436 18, 436 63, 435 2, G01N 3100

Patent

active

053895495

ABSTRACT:
Reagents for enabling leukocytes to be classified and counted lyse erythrocytes and act on leukocytes to stabilize them are disclosed. The reagents contain polyoxyethylene-based surfactants, and may contain hyperosmotic or hypoosmotic agents and solubilizing agents. One embodiment of the invention contains surfactants selected from the anionic and nonionic polyoxyethylene-based surfactants having 18-30 repeating oxyethylene units per molecule. A second embodiment of the invention contains nonionic polyoxyethylene surfactants only, having 20-100 repeating oxyethylene units per molecule, does not employ either saponin or quaternary ammonium compounds, and provides for adjustment of the pH of the resulting solutions. The invention allows leukocytes to be classified into as many as five types, each type to be quantified, and enables the detection of abnormal cells, when used in conjunction with particulate analysis flow cytometers employing the RF method and the DC method.

REFERENCES:
patent: 2656508 (1953-10-01), Coulter
patent: 3390326 (1968-06-01), Imadate
patent: 3502974 (1970-03-01), Coulter
patent: 3741875 (1973-06-01), Ansley
patent: 3836849 (1974-09-01), Coulter
patent: 4099917 (1978-07-01), Kim
patent: 4185964 (1980-01-01), Lancaster
patent: 4286963 (1981-09-01), Ledis et al.
patent: 4485175 (1984-11-01), Ledis
patent: 4488175 (1984-12-01), Netravali
patent: 4502613 (1990-02-01), Chang et al.
patent: 4506018 (1985-03-01), North, Jr.
patent: 4528274 (1985-07-01), Carter et al.
patent: 4529705 (1985-07-01), Larsen
patent: 4617275 (1986-10-01), Matsuda et al.
patent: 4637986 (1987-01-01), Brown et al.
patent: 4745071 (1988-05-01), Lapicola et al.
patent: 4751179 (1988-07-01), Ledis et al.
patent: 4962038 (1990-10-01), Carter et al.
patent: 5116539 (1992-05-01), Hamaguchi et al.
I. Kurokawa, et al. "Experience of the use of Toa Automatic Blood cell counter": Rinsho Byori (Clinical Pathology) 16, 251-55, 1968.
I. Kurokawa, et al. Rinsho Kensa (Clinical Testing) 11, 148-151. 1967.
K. Shintani, "Problems with Leukocyte counting", Rensho Kensa, (Clinical Testing) 12, 900-905, 1968.
Hamaguchi, et al., Chemical Abstracts CA 111:228524v (Dec. 1989) abstract of WO 09504 PCT/JP88/00514, priority doc U.S. #07/926,085.
Y. Takamori, et al. "On Factors Causing Variations in Leukocyte Size-Frequency Distribution and Their Effects on Leukocyte Counts" MCC News, 34, Dec. 21, 1969.
Helleman, P. W., et al. Scand. J. Haematology, 6, 160-165, 1969.

Affiliated with

Also associated with

Rating

Method for classifying leukocytes and a reagent used therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for classifying leukocytes and a reagent used therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for classifying leukocytes and a reagent used therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-287397