Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Concentration – separation – or purification of micro-organisms
Reexamination Certificate
2003-06-23
2009-12-01
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Concentration, separation, or purification of micro-organisms
C436S063000, C436S172000
Reexamination Certificate
active
07625730
ABSTRACT:
There is provided a method for classifying and counting leukocytes with abnormal DNA amount, which comprises:(1) a step of staining cells in a sample obtained from a hematological sample by treatment with a hemolytic agent to lyse erythrocytes, with a fluorescent dye which can make a difference in the fluorescence intensity at least among mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes;(2) a step of introducing the sample containing the stained cells into a flow cytometer to measure scattered light and fluorescence of the respective cells;(3) a step of classifying leukocytes and coincidence cells/platelet clumps utilizing a difference in the intensity of a scattered light peak and a difference in the scattered light width;(4) a step of classifying and counting mature leukocytes, leukocytes with abnormal DNA amount and immature leukocytes, utilizing a difference in the scattered light intensity and a difference in the fluorescence intensity of leukocytes classified in the step (3).
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Konishi Aya
Mizukami Toshihiro
Mori Yusuke
Nakazawa Yukie
Tsuji Tomohiro
Gitomer Ralph
Sughrue & Mion, PLLC
Sysmex Corporation
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