Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Particle count or volume standard or control
Patent
1995-06-05
1997-10-14
Warden, Jill
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Particle count or volume standard or control
436 17, 436 63, 436164, 436166, 436171, 436175, G01N 3100, G01N 3348
Patent
active
056771835
ABSTRACT:
A method for classifying and counting leukocytes which includes the steps of (i) adding a first reagent used for classifying leukocytes into four groups which contains, (a) at least one ionic surfactant in a sufficient amount to lyse erythrocytes and to damage a part of cell membrane of leukocytes, (b) at least one organic compound having an anionic group in a sufficient amount to bond with a cationic component present in leukocytes to give morphological differences between leukocytes, (c) a nonionic surfactant, and (d) a buffer for adjusting pH, to a part of a blood sample to determine information on the cell size and morphological features in order to classify leukocytes into four groups consisting of three groups corresponding to lymphocytes, mononuclear cells and eosinophils and one group corresponding to neutrophils and basophils; (ii) adding a second reagent used for measuring basophils to another part of the blood sample to determine information on at least the cell size in order to classify basophils, and (iii) classifying leukocytes based on the information obtained in the steps (i) and (ii) and counting.
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JP 03020667 Abstract, WPIDS Accession. No. 91-070681, Jan. 29, 1991.
Akai Yasumasa
Hyousa Yoshihiro
Kouzuki Chihiro
Sakata Takashi
Takarada Kaoru
TOA Medical Electronics Co., Ltd.
Wallenhorst Maureen M.
Warden Jill
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