Method for classifying and counting immature leukocytes

Details Method for classifying and counting immature leukocytes Method for classifying and counting immature leukocytes

1997-11-17

1999-09-28

Wallenhorst, Maureen M.

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

436 8, 436 17, 436 63, 436164, 436166, 436172, 435 29, 435 34, 435 39, G01N 3100

Patent

active

059587768

ABSTRACT:
A hematological sample is treated with a hemolytic agent that maintains immature leukocytes in a viable state and damages other leukocytes. The damaged leukocytes are stained with a fluorochrome which can stain damaged cells. At least one kind of scattered light and at least one kind of fluorescence of the treated and stained blood cells are measured to classify and count leukocytes based on the intensities of the scattered light and the fluorescence. Highly precise measurement of immature leukocytes and simultaneous classification and counting of normal leukocytes are achieved.

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H. M. Shapiro et al.,: "Combined Blood Cell Counting and Classification with Fluorochrome Stains and Flow Instrumentation". The Journal of Histochemistry and Cytochemistry, vol. 24, No. 1, Jan. 1976.

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