Method for classification of pixels into groups according to the

Details Method for classification of pixels into groups according to the Method for classification of pixels into groups according to the

1997-08-25

1998-11-10

Elliott, George C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

359368, 252582, C12Q 168, G02B 2116, F21V 908

Patent

active

058342036

ABSTRACT:
A method of classification of pixels into groups of pixels according to their association with a single fluorophore or a combination of fluorophores selected from a plurality of fluorophores, each of the fluorophores having characterizing excitation and emission spectra and specifying excitation and emission peaks, the method comprising the steps of (a) providing a plurality of pairs of wide-band excitation filters and wide-band emission filters; (b) exciting fluorophores of each of the pixels with light filtered through one of the wide-band excitation filters, and recording emitted light intensity as retrieved after passing through its paired emission filter; (c) repeating step (b) for all of the plurality of pairs of filters, such that each of the pixels is representable by a vector of a plurality of dimensions, the number of dimensions being equal to the number of the plurality of pairs of filters; (c) using an algorithm for evaluating the presence of each of the plurality of fluorophores in each of the pixels, thereby classifying each of the pixels into a group of pixels according to its association with a single fluorophore or combination of fluorophores.

REFERENCES:
Speicher et al, "Computer Image Analysis of Combinatorial Multi-Fluor FISH", Bioimaging, 4:52-64, 1996.
Veldman et al, "Hidden Chromosome Abnormalities in Haematological Malignancies Detected by Multicolor Spectral Karyotpying", Nature Genetics, 15: 406-410, 1997.

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