Method for chromosome classification by decorrelaiton statistica

Chemistry: molecular biology and microbiology – Apparatus – Including measuring or testing

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359368, 252582, C12M 134, G02B 2116, F21V 908

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059121653

ABSTRACT:
A method and hardware for chromosome classification by decorrelation statistical analysis to provide color (spectral) karyotypes and to detect chromosomal aberrations. The method and hardware employ a set of N decorrelation matched filters for chromosome classification. The N decorrelation matched filters are dedicated for extracting decorrelated spectral data from chromosome samples painted according to a specific experimental protocol. The N decorrelation matched filters being described by: ##EQU1## where V.sub.ik min equals minimum V.sub.ik over all i, and V.sub.ik max equals maximum V.sub.ik over all i, and wherein N is an integer greater than two.

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Ploem "Fluorescence Microscopy" in Light Microscopy in biology; a practical approach Lacey Ed. IRL Press, 1989.
Reid et al. Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc. Natl. Acad. Sci. USA vol. 89 pp. 1388-1392, 1992.

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