Method for chromatographic separation of synthetic phosphorothio

Details Method for chromatographic separation of synthetic phosphorothio Method for chromatographic separation of synthetic phosphorothio

1991-10-18

1993-02-02

Rollins, John W.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536 553, 536 2712, 935 19, C07B 6300, C07H 2104, C07H 106

Patent

active

051838850

ABSTRACT:
A method is provided for the preparation and/or analysis or synthetic phosphorothioate and -dithioate oligonucleotides. In particular, the method permits separation of fully sulfurized phosphorothioate or -dithioate oligonucleotides from incompletely sulfurized defect species on strong-anion exchange HPLC columns using concentration gradients of novel "soft base" anionic eluents, such as bromide, thiocyanate, and the like.

REFERENCES:
LaPlanche et al., Nucleic Acids Research, vol. 14, pp. 9081-9093 (1986).
Haddad and Jackson, Ion Chromatography (Elsevier, Amsterdam, 1990), pp. 1-25, 29-47, and 195-207.
Pearson, J. Amer. Chem. Soc., vol. 85, pp. 3533-3539 (1963).
Chemical Abstracts 112(11):94794t. Maisano et al. BioChromatography 4(5):279-281, 1989.
Matsukura et al. Proc. Natl. Acad. Sci. 84:7706-7710, 1987.

Affiliated with

Also associated with

Rating

Method for chromatographic separation of synthetic phosphorothio does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for chromatographic separation of synthetic phosphorothio, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for chromatographic separation of synthetic phosphorothio will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-7433