Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2008-01-08
2010-11-16
Hobbs, Lisa J (Department: 1657)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S358000, C435S029000, C435S378000, C435S404000, C424S093700
Reexamination Certificate
active
07833785
ABSTRACT:
The invention provides a novel method of dissociating anchorage independent and dependent cell aggregates. The invention also includes the cells resulting from the methods of the invention and the use of the cells in various applications requiring the generation of a single cell suspension.
REFERENCES:
patent: 2002/0198928 (2002-12-01), Bukshpan et al.
patent: 2003/0040104 (2003-02-01), Barbera-Guillem
patent: WO 2004/013315 (2004-02-01), None
Reynolds, B.A., and Weiss, S. 1996. Clonal and Population Analyses Demonstrate That an EGF-Responsive Mammalian Embryonic CNS Precursor Is a Stem Cell. Developmental Biology 175:1-13.
Allen, C.N., Brady, R., Swann, J., Hori, N., and Carpenter, D.O. 1988. N-Methyl-D-aspartate (NMDA) receptors are inactivated by trypsin. Brain Research 458:147-150.
Lee, S.H., Lumelsky, N., Studer, L., Auerbach, J.M., and McKay, R.D. 2000. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nature Biotechnology 18:675-679.
Murayama, A., Matsuzaki, Y., Kawaguchi, A., Shimazaki, T., and Okano, H. 2002. Flow Cytometric Analysis of Neural Stem Cells in the Developing and Adult Mouse Brain. Journal of Neuroscience Research 69:837-847.
Martin, F.C., and Wiley, C.A. 1994. An alternative method for obtaining high viability cell suspensions from neonatal mouse brain. Journal of Neuroscience Methods 55:99-104.
Reynolds, B.A., and Weiss, S. 1992. Generation of Neurons and Astrocytes from Isolated Cells of the Adult Mammalian Central Nervous System. Science 255:1707-1710.
Stingl, J., Emerman, J.T., and Eaves, C.J. 2005. Enzymatic Dissociation and Culture of Normal Human Mammary Tissue to Detect Progenitor ActivityfromMethods in Molecular Biology 290:249-263.
Caldwell, M.A., He, X., Wilkie, N., Pollack, S., Marshall, G., Wafford, K.A., and Svendson, C.N. 2001. Growth factors regulate the survival and fate of cells derived from human neurospheres. Nature Biotechnology 19:475-479.
Avellana-Adalid, V., Nait-Oumesmar, B., Lachapelle, F., and Baron-Van Evercooren, A. 1996. Expansion of Rat Oligodendrocyte Progenitors Into Proliferative “Oligospheres” That Retain Differentiation Potential. Journal of Neuroscience Research 45:558-570.
Torres, F.X., Mackowiak, P.G., Brown, R.D., Linden, M.D., and Zarbo, R.J. 1995. Comparison of two methods of mechanical disaggregation of scirrhous breast adenocarcinomas for DNA flow cytometric analysis of whole cells. American Journal of Clinical Pathology 103(1):8-13.
Sen, A., Kallos, M.S., Behie, L.A. 2004. New Tissue Dissociation Protocol for Scaled-up Production of Neural Stem Cells in Suspension Bioreactors. Tissue Engineering 10(5/6):904-913.
Youn, B.S., Sen, A., Kallos, M.S., Behie, L.A., Girgis-Gabardo, A., Kurpios, N., Barcelon, M., Hassell, J.A. 2005. Large-Scale Expansion of Mammary Epithelial Stem Cell Aggregates in Suspension Bioreactors. Biotechnology Program 21(3):984-993.
Sen, A., Kallos, M.S., Behie, L.A., Experimental Techniques for the Dissociation of Cellular Aggregates in the Large-Scale Production of Mammalian Neural Stem Cells in Suspension Bioreactors, Abstract, 50thCanadian Chemical Engineering Conference, Oct. 15-18, 2000, Montreal, QC.
Sen, A., Kallos, M.S., Behie, L.A., A New Protocol for the Dissociation of Mammalian Neural Stem Cell Aggregates, Abstract, Keystone Symposia (From Stem Cells to Therapy), Steamboat Springs, Colorado, Mar. 29-Apr. 3, 2003.
Kallos et al., Extended Serial Passaging of Mammalian Neural Stem Cells in Suspension Bioreactors, 1999, Biotechnology and Bioengineering, vol. 65, No. 5, p. 589-599.
Behie Leo A.
Kallos Michael S.
Sen Arindom
Bereskin & Parr LLP / S.E.N.C.R.L., s.r.l.
Gravelle Micheline
Hobbs Lisa J
LandOfFree
Method for chemical dissociation of cells from physical... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for chemical dissociation of cells from physical..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for chemical dissociation of cells from physical... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4247789