Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2006-05-11
2009-11-24
Gitomer, Ralph (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
Reexamination Certificate
active
07622273
ABSTRACT:
The invention provides a simple and quick protocol for chemical treatment, enzymatic or chemical digestion, and subsequent identification of proteins or polypeptides on a protein chip such as protein chip arrays. The chemical treatment includes denaturation, reduction and alkylation while the enzymatic digestion includes deglycosylation, dephosphorylation, and digestion by various proteases. The proteins or polypeptides can also be digested by using various chemicals that are known to induce proteolysis. All reactions are carried out sequentially on chip. Subsequent peptide mass fingerprinting or product ion searches allow the identification of specific peptides, which can be correlated to proteins. The methods of the present invention can be used to analyze biological samples such as urine and plasma and to identify biomarkers in diseased states. The methods of the present invention allow complete on chip treatment, which can be used for rapid protein identification and structural characterization of heavily posttranslationally modified proteins.
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Gitomer Ralph
Goudreau Gage Dubuc
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