Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Reexamination Certificate
2007-01-16
2007-01-16
Gitomer, Ralph (Department: 1655)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
C436S089000
Reexamination Certificate
active
10479843
ABSTRACT:
Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.
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Hamon Christian
Johnstone Robert
Mohammed Abdul Karim Abed
Neumann Thomas
Thompson Andrew
Buchanan Ingersoll & Rooney P.C.
Electrophoretics Limited
Gitomer Ralph
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