Method for centrifugal particle separation, particularly for use

Details Method for centrifugal particle separation, particularly for use Method for centrifugal particle separation, particularly for use

1997-03-26

1999-07-13

Reifsnyder, David A.

Liquid purification or separation

Processes

Separating

210782, 210787, 210805, 422 72, 422101, 436 45, 494 37, B04B 502

Patent

active

059222115

ABSTRACT:
The invention relates to a method for centrifugal particle separation, particularly in the biological sector, wherein a sample (26) to be fractionated is introduced into a centrifuge vessel (1) via a cannula (5), the free end (51) of which extends to point (1') of a centrifuge vessel (1) which sharply tapers toward tip (1') to minimize the undesirable effects of the Coriolis force, with a gradient solution (7) having a density that increases continuously or step by step then being introduced via cannula (5), with particles from sample (26) migrating due to the action of equilibrium and/or sedimentation centrifugation into gradient solution (7), and with a pressure fluid being introduced into the interior of sealed centrifuge vessel (1) via an additional cannula (17) after a predetermined centrifugation period to expel gradient solution (7) containing the fractionated particles of sample (26) via cannula (5).

REFERENCES:
patent: 4648863 (1987-03-01), Nees
patent: 5663051 (1997-09-01), Vlasselaer

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