Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Reexamination Certificate
2008-07-15
2008-07-15
Carlson, Karen Cochrane (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
Reexamination Certificate
active
07399610
ABSTRACT:
The present invention provides a simple cell-free protein synthesis method capable of affording synthesis of a protein in a high amount in a short time at a low cost. A method for cell-free protein synthesis using an extract derived from an insect cell, the method comprising removing a component which can pass through a semipermeable membrane through the semipermeable membrane while maintaining synthesis reaction, thereby to continuously synthesize a protein. Preferably, an mRNA is additionally supplied while said synthesis reaction is maintained. Further, said insect cell is preferably an established culture cell derived fromTrichoplusia niovum cell.
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Alexander S. Spirin, et al., “A Continuous Cell-Free Translation System Capable of Producing Polypeptides in High Yield”, Science, vol. 242, Nov. 25, 1988, pp. 1162-1164.
Hanafusa Nobuhiro
Kobayashi Shin-ichiro
Shikata Masamitsu
Carlson Karen Cochrane
Cheng Law Group PLLC
Rooke Agnes B.
Shimadzu Corporation
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