Method for cell culture

Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Method of detaching cells – digesting tissue or establishing...

Reexamination Certificate

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Reexamination Certificate

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07094602

ABSTRACT:
A method for detaching a carrier for cell culture from cultured cells formed on a surface of said carrier, which includes the step of bringing the carrier for cell culture into contact with a compound represented by the following formula (I) or a polyphosphoric acid or a salt thereof: MO3P-L11-PO3M wherein L11represents a divalent bridging group; and M represents a hydrogen atom or a cation. The carrier preferably includes a calcium alginate layer and a cell adhesion gel layer.

REFERENCES:
patent: 5292525 (1994-03-01), Brenden et al.
patent: 6821107 (2004-11-01), Hara et al.
Esser S et al. “Copper complexation index as a polarographic summation parameter for the determination of chelating agents in water”. Fresenius J. Anal. Chem., 2000, 368(2-3): 250-5. Entire document.

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