Method for carrying out enzyme assays

Details Method for carrying out enzyme assays Method for carrying out enzyme assays

1986-01-28

1989-07-18

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 25, 435 27, 435 28, 436826, G01N 33535, C12Q 126, C12Q 128

Patent

active

048493422

ABSTRACT:
Peroxidatively active enzyme assays have a variety of clinical and industrial applications. Such assays are based on color development resulting from the addition of the appropriate substrate to the enzyme-containing medium. The stability of the said substrate and the extent of appearance of color are two serious limitations in enzyme assays. A method is disclosed herein for carrying out peroxidatively active enzyme assays when the enzyme is in free solution or in cell-bound form. Stable chemical compositions are also disclosed for use as substrates for carrying out such method.

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Inagaki, Chemical Abstracts, 92:2755y (1980).
Sugiura, Chemical Abstracts, 91:86919v (1979).
Thorpe et al., Clin. Chem., 31, 8, 1335-1345 (1985).

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