Method for calibrating antibiotic disk diffusion testing of...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

Reexamination Certificate

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C702S019000

Reexamination Certificate

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07465559

ABSTRACT:
A method for calibrating antimicrobic susceptibility testings of microorganisms comprises the steps of creating a histogram with a high response side and a low response side of isolates from a microbial species, which may contain resistant strains against an antimicrobial agent, calculating from the high response side of the histogram at least one statistical parameter, and defining a limit for susceptibility interpretation and comparative analysis of antimicrobic resistance, which is based on the statistical parameter. This limit separates susceptible strains from resistant strains against an antimicrobial agent.

REFERENCES:
APMIS, vol. 99, 1991, Göran Kronvall et al: “Compaison of 30 ug and 120 ug gentamicin disks for the prediction of gentamicin resistance inEnterococcus faccalis”, pp. 887-892, see pp. 889-891.
Journal of Clinical Microbiology, vol. 16, No. 5, 1982, Göran Kronvall: “Analysis of a Single Reference Strain for Determination of Gentamicin Regression Line Constants and Inhibition Zone Diameter Breakpoints in Quality Control of Disk Diffusion Antibiotic Susceptibility Testing”, pp. 784-793.
Antimicrobial Agents and Chemotherapy, vol. 32, No. 10, 1988, Göran Kronvall et al: “Laboratory-and Species-Specific Interpretive Breakpoints for Disk Diffusion Tests of Chloramphenicol Susceptibility of Haemophilus influenzae”, pp. 1484-1489.
APMIS, vol. 99, 1991, Göran Kronvall et al: “Antibiotic disk diffusion testing revisted, Single strain regression analysis”, pp. 295-306.
Scand J. Infect. Dis. Suppl., vol. 105, 1997, Gunnar Kahlmeter et al: “Antimicrobial Susceptibility Testing in Sweden. IV. Quality Assurance”, pp. 24-31.
Scand J. Infect. Dis. Suppl., vol. 105, 1997, Barbro Olsson-Liljequist et al: “Antimicrobial Susceptibility Testing in Sweden. III. Methodology for Susceptbility Testing”, pp. 13-23.
Scand J. Infect. Dis. Suppl., vol. 105, 1997, Signe Ringertz et al: “Antimicrobial Susceptibility Testing in Sweden. II. Species-related Zone Diameter Breakpoints to Avoid Interpretive Errors and Guard Against Unrecognized Evolution of Resistance”, pp. 8-12.

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