Chemistry: analytical and immunological testing – Including chromatography
Patent
1988-07-27
1991-10-15
Lacey, David L.
Chemistry: analytical and immunological testing
Including chromatography
436178, 436815, 436901, 210656, 2101982, G01N 3002
Patent
active
050574370
ABSTRACT:
Biological fluid test samples are analyzed for a broad spectrum of drugs, including benzodiazepines, amphetamines, tricyclic antidepressants and opiates, in a single isocratic analysis using a chromatographic column system containing three analytical columns--an anion exchange column, a reversed phase column and a cation exchange column. A pre-column is also included to purge the sample of salts, proteins, peptides and hydrophilic anions. Carrier liquids containing acetonitrile at various strengths are used for distribution of the various drugs among the columns, elution of the drugs from the columns, and column purging and conditioning. The system readily lends itself to automation, automatic periodic sampling, and component identification and quantification.
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Bio-Rad Laboratories, Inc.
Lacey David L.
Snay Jeffrey R.
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