Method for blood film preparation

Coating processes – Medical or dental purpose product; parts; subcombinations;... – Particulate or unit-dosage-article base

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Details

427240, A01N 102, B05C 1102, G01N 128

Patent

active

040370038

ABSTRACT:
Sample preparation apparatus for forming a thin layer of diluted blood specimen on a substrate by centrifugal force includes a sample probe having coaxial blood and diluent conduits coupled to respective reversibly drivable peristaltic pumps having forward operation intakes connected to a diluent supply. A one-way clutch limits the diluent pump to forward operation and the pumps are geared to produce a blood-to-diluent displacement ratio of 2:1. A substrate holder is mounted on a carriage for selective insertion into and removal from a sealed centrifuge chamber in which it is spun at a high angular rate by a drive motor. Means are provided to form a curtain of flowing liquid within the chamber surrounding the spinning substrate to collect spun-off blood particles and aerosol. The liquid forming the curtain is drained by an exhaust pump which operates after spinning has ceased so as to create a partial vacuum for draining off residual aerosol prior to unsealing the centrifuge chamber.

REFERENCES:
patent: 2412099 (1946-12-01), Sender
patent: 2580131 (1951-12-01), Rowell
patent: 2941499 (1960-06-01), Gutzmer
patent: 2952557 (1960-09-01), Charron
patent: 3280792 (1966-10-01), Heyde
patent: 3705048 (1972-12-01), Staunton
patent: 3906890 (1975-09-01), Amos et al.

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