Method for biological cell and particulate analysis

Optics: measuring and testing – For size of particles – By particle light scattering

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356338, 356244, 356440, 2502222, 250574, G01N 1502

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active

055813496

ABSTRACT:
An analytical device for detecting and analyzing the population of neutral or electrically-charged solutes, including providing a light source for producing a collimated beam, providing a solution vessel containing the sample solution to be analyzed, directing the light beam through the sample solution to thereby produce scattered light, a photomultiplier or a photon counter for detecting the scattered light, said photomultiplier being positioned at an angle to receive the scattered light from the collimated beam, an analog-to-digital converter communicating with the photomultiplier, converting the electrical signals output into a digital output, a digital correlator for receiving the digital output and to calculate a time autocorrelation function of the motion of the solutes in the fluid medium, immersing two electrodes in the solution vessel, generating an electrical field between the two electrodes, positioning the electrodes such that a field gradient is created between the electrode, and such that the light beam partially impinges on one of the electrodes to create a heterodyne effect, and an electrical field generator connected to said electrodes, producing an oscillating electrical signals, that can polarize solutes in the solution, the electrical signal generated at a frequency of 0 to 1,000 million hertz.

REFERENCES:
patent: 3870612 (1975-03-01), Flygare et al.
patent: 4233664 (1980-11-01), Grandclamp
patent: 5298967 (1994-03-01), Wells
Kenneth S. Schmitz, Dept. of Chemistry, U of MO-Kansas City Article from Chemical Physics Letters, vol. 63, No. 2, May 15, 1979 titled "Quasielastic Light Scattering by Biopolymers, Center of Mass Motion of DNA in the Presence of a sinusoidal electric field."
B. R. Ware, Dept of Chemistry, Harvard University, article from Advances in Colloid and Interface Science, titled "Electrophoretic Light Scattering".

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