Method for B cell typing of total human lymphocyte sample

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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424 11, 435 29, 436513, 436519, 436548, 436808, 436821, G01N 3353, G01N 33554, G01N 33577

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active

046578528

ABSTRACT:
An HLA-DR typing test based on lymphocytotoxicity in which a vital dye-labeled total human lymphocyte sample, such as a sample of peripheral blood lymphocytes, is incubated with HLA-DR antisera, a monoclonal antibody against T cells, and complement and the DR type is determined based upon the resultant cytotoxicity as measured by the fluorescence of B cells surviving the incubation.

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Bland, et al., Joint Report, Technique of Separation, Storage and DR Test of B Lymphocytes, Addenbrooke's Hospital, Cambridge, England.

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