Method for automated isolation of fractions in multichannel...

Chemistry: analytical and immunological testing – Optical result

Reexamination Certificate

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C436S161000, C436S172000, C436S177000, C436S178000, C422S070000, C422S082050, C422S082080, C422S082090, C422S105000, C073S061580, C204S451000, C204S452000, C204S455000, C204S457000, C204S601000, C204S602000, C204S603000, C204S607000, C210S198200, C210S656000, C356S344000

Reexamination Certificate

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06984525

ABSTRACT:
A method to collect a plurality of target zones from a plurality of channels. The method includes introducing a plurality of compound mixtures into a plurality of microchannels on a microfabricated chip or a capillary tube. Optical signal measured at a detector window provides a signal of the position of a target zone. When a target zone has migrated to the location of a detector window, the detector senses an optical signal from the target zone and signals the system to disconnect a driving force from that channel. Once a plurality of zones in a plurality of channels are aligned, the driving force is reconnected and the zones are coeluted into a collection container.

REFERENCES:
patent: 5541420 (1996-07-01), Kambara
patent: 6387235 (2002-05-01), Irie et al.
patent: 6660149 (2003-12-01), Karger et al.
O. Muller et al., “Design of a High-Precision Fraction Collector for Capillary Electrophoresis”, Anal. Chem., 1995, vol. 67, pp. 2974-2980.
M. Minarik et al., “Design of a Fraction Collector for Capillary Array Electrophoresis”, Electrophoresis 2002, vol. 23, pp. 35-42.
T. Irie et al., “Automated DNA Fragment Collection by Capillary Array Gel Electrophoresis in Search of Differentially Expressed Genes”, Electrophoresis 2000, vol. 21, pp. 367-374.

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