Chemistry: analytical and immunological testing – Optical result – With fluorescence or luminescence
Patent
1994-12-05
1996-09-17
Alexander, Lyle A.
Chemistry: analytical and immunological testing
Optical result
With fluorescence or luminescence
436 91, 436 94, G01N 3368
Patent
active
055567908
ABSTRACT:
An automated DNA sequencing apparatus and method is disclosed in which a laser beam having a predetermined wavelength is sequentially focused on a plurality of lanes of DNA fragments migrating in a polyacrylamide gel and in which the DNA fragments have been tagged with fluorescent compounds. The resulting fluorescent light given off by each of the tagged DNA fragments is collected and diffracted by means of an acousto-optic tunable filter and the level of the diffracted light is then detected and analyzed in order to determine the DNA sequences of the analyzed DNA fragments. The automatic DNA sequencing system of the present invention operates under computer control to repeat a series of measurements of the fluorescent data at a multiple number of wavelengths such that the entire spectrum of fluorescence emissions from all four fluorescent tagged DNA bases can be measured at any number of wavelengths.
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